Journal: Heliyon

Article Title: Atrial fibrillation recurrence after catheter ablation is associated with RAD51 and p63 proteins

doi: 10.1016/j.heliyon.2024.e32874

Figure Lengend Snippet: Images of the microarrays from a representative slide of Explorer antibody microarray (ASB600, Full Moon Biosystems, USA) with 656 antibodies per slide in two replicates for each antibody. Enlarged images of individual samples illustrate the differences in the levels of RAD51 in the serum of a patient with compensated AF of the subgroup M1 (A) versus that in the serum of a patient with AF recurrence of the subgroup M2 (B).

Article Snippet: Images of the microarrays from a representative slide of Explorer antibody microarray (ASB600, Full Moon Biosystems, USA) with 656 antibodies per slide in two replicates for each antibody.

Techniques: Microarray

Journal: Heliyon

Article Title: Atrial fibrillation recurrence after catheter ablation is associated with RAD51 and p63 proteins

doi: 10.1016/j.heliyon.2024.e32874

Figure Lengend Snippet: Images of the microarrays from a representative slide of Explorer antibody microarray (ASB600, Full Moon Biosystems, USA) with 656 antibodies per slide in two replicates for each antibody. Enlarged images of individual samples illustrate the differences in the levels of p63 in the serum of a patient with compensated AF of the subgroup M1 (A) versus that in the serum of a patient with AF recurrence of the subgroup M2 (B).

Article Snippet: Images of the microarrays from a representative slide of Explorer antibody microarray (ASB600, Full Moon Biosystems, USA) with 656 antibodies per slide in two replicates for each antibody.

Techniques: Microarray

Journal: Clinical Proteomics

Article Title: Current applications of antibody microarrays

doi: 10.1186/s12014-018-9184-2

Figure Lengend Snippet: Review of planar antibody microarray technologies and their applications in the field of proteomics. Images were adopted from Servier Medical Art by Servier ( http://www.servier.com/Powerpoint-image-bank ) and modified by the authors under the following terms: CREATIVE COMMONS Attribution 3.0 Unported (CC BY 3.0)

Article Snippet: Fullmoon Biosystems Inc , Prostate cancer , Phosphorylation-specific antibody microarray , 95 , 24009409.

Techniques: Microarray, Modification

Journal: Clinical Proteomics

Article Title: Current applications of antibody microarrays

doi: 10.1186/s12014-018-9184-2

Figure Lengend Snippet: Antibody microarray summary

Article Snippet: Fullmoon Biosystems Inc , Prostate cancer , Phosphorylation-specific antibody microarray , 95 , 24009409.

Techniques: Microarray, Phospho-proteomics, Infection, Biomarker Discovery, Virus

Journal: Experimental Biology and Medicine

Article Title: Annona atemoya leaf extract ameliorates cognitive impairment in amyloid-β injected Alzheimer’s disease-like mouse model

doi: 10.1177/1535370219886269

Figure Lengend Snippet: Molecular mechanisms responsible for anti-AD effects of AAL extract. (a) HT22 cells were exposed to H2O2 in the absence or presence of AAL extract (50 μg/mL) for 6 h. Antibody microarray assay was performed using the phospho-specific antibody microarray slide (Full Moon BioSystems). For data acquisition, GenePix 4100A scanner (Axon Instrument, USA) was used. The normalization data were analyzed using Genowiz 4.0™ (Ocimum Biosolutions). The phosphorylation ratio was calculated and represented as fold changes of indicated phosphoproteins after H2O2 treatment normalized to total protein expression (upper panel). Total protein quantification is shown (lower panel). (b) HT22 cells were exposed to H2O2 with or without various concentrations of AAL extract (0, 12.5, 25, or 50 μg/mL) for 6 h. Cell lysates were prepared from HT22 cells and equal amounts of protein were subjected to Western blotting using anti-phospho-CaMK2 β/ν/δ, GRK2, EGFR, Myc, FER, caveolin-1, NFκB p65, and MLRN 2 antibodies to validate the Ab microarray. (c) Protein extracts were prepared from hippocampal tissues in an Aβ-induced AD mouse model. Vehicle or various concentrations of AAL extract (50, 100, or 200 mg/kg) were administered to Aβ mice for 23 days. Western blotting was performed for anti-phospho-EGFR and phospho-GRK2. The validity of the two phospho-antibodies was determined using pre-stained protein marker (Bio-Rad). GAPDH was used as an internal control. Shown blots are representative results from three independent experiments.

Article Snippet: Molecular mechanisms responsible for anti-AD effects of AAL extract. (a) HT22 cells were exposed to H 2 O 2 in the absence or presence of AAL extract (50 μg/mL) for 6 h. Antibody microarray assay was performed using the phospho-specific antibody microarray slide (Full Moon BioSystems).

Techniques: Microarray, Phospho-proteomics, Expressing, Western Blot, Staining, Marker, Control

Journal: Cancer research

Article Title: Macrophage-derived IL-1β and TNF-α regulate arginine metabolism in neuroblastoma

doi: 10.1158/0008-5472.CAN-18-2139

Figure Lengend Snippet: A) Non-metric multi-dimension scaling of Stage I (RED) and Stage IV (BLUE) tumours at diagnosis shows distinct proteomic profiles for these two stages of tumours B) Proteomic analysis of Stage I and IV tumours identifies significantly higher expression of CD14 and CD15 in Stage IV tumours C) Immunohistochemical staining of sections from neuroblastomas showing infiltration of CD14+ (upper) and CD15+(lower) myeloid cells. Representative sections shown of n=27, tissue microarray D) Histoscores of CD14 and CD15 staining in neuroblastoma tissue microarrays of n=27 tumours E) CD14+ monocytes from healthy donors co-cultured with sorted GD2+ tumour cells from patients upregulate CD68 expression (upper). Minimal CD206 upregulation was seen. Representative flow cytometry shown (n=5) F) CD14+ monocytes from healthy donors co-cultured with neuroblastoma have decreased arginase activity, as assessed by conversion of ornithine to urea in a colorimetric assay. (n=3) G) Immunohistochemical staining of sections from neuroblastomas showing infiltration of CD68+ macrophages. Representative sections shown of n=27, tissue microarray

Article Snippet: Human stage I neuroblastoma tissue samples (n=13), human stage IV neuroblastoma biopsies (n=9) were analysed using scioDiscover antibody microarrays (Sciomics) which targets 900 cancer-related proteins ( 16 ).

Techniques: Biomarker Discovery, Expressing, Immunohistochemical staining, Staining, Microarray, Cell Culture, Flow Cytometry, Activity Assay, Colorimetric Assay

Journal: Blood Advances

Article Title: Citron Rho-interacting kinase silencing causes cytokinesis failure and reduces tumor growth in multiple myeloma

doi: 10.1182/bloodadvances.2018028456

Figure Lengend Snippet: CIT is highly expressed in MM patients. (A) Significant increase in CIT in primary BM-derived CD138+ MM plasma cells compared with healthy subjects by proteomic analysis using the Antibody Microarray (BD Clontech). (B) CIT gene expression levels in healthy control, newly diagnosed, and relapsed MM patients are shown. (C) Expression of CIT in BM biopsy specimens from MM patients (n = 11) and healthy donors (normal bone marrow [NMB]; n = 6) was detected by immunohistochemistry. Representative slides are shown for each group (original magnification ×20 [left] and ×60 [right]). (D) CIT+ cells in immunohistochemistry-stained slides were quantified using ImageJ software (P = .02). (E) Using CIT median gene expression value as a bifurcating point in GSE2658, Kaplan-Meier survival proportions analysis of patient outcome data demonstrates high levels of CIT gene expression associated with inferior OS (P = .01).

Article Snippet: Protein microarray An Antibody Microarray (BD Clontech, Palo Alto, CA) was conducted in 12 MM patients’ BM plasma cells.

Techniques: Derivative Assay, Microarray, Expressing, Immunohistochemistry, Staining, Software

Journal: International Journal of Proteomics

Article Title: Cladribine and Fludarabine Nucleoside Change the Levels of CD Antigens on B-Lymphoproliferative Disorders

doi: 10.1155/2010/964251

Figure Lengend Snippet: Expression of CD antigens on Raji and MEC2 cells treated with purine analogs (1 μ M, 24 hours) analyzed using microarrays. (a) Raji + CdA, (b) Raji + FdA, and (c) MEC2 + CdA. Expression profiles were generated from the dot patterns of cells captured on microarrays; control (grey bars), drug-treated (black bars). CD antigens are labelled on the abscissa, average dot intensities on an 8-bit greyness scale (1–256) on the ordinate. Mean dot intensities are plotted with standard error bars ( n = 3). Asterisks indicate significant ( P < .05) changes.

Article Snippet: DotScan TM microarrays were obtained from Medsaic (Eveleigh, Australia).

Techniques: Expressing, Generated, Control